Figure 1 Stratified pavement type epithelium consisting of approximately 5 to 6 cell layers. Blood vessels (v) in the conjunctivae chorion. Sclera (e) with multiple layers of dense connective tissue (G1: M3-M5-HE×100)
Figure 2 The absence of conjunctivae epithelium was observed at the site of application (G2-M1- HE×200)
Figure 3 Conjunctiva epithelium without cell differentiation. Subepithelial edema (G2-M2-HE×200)
Figure 4 Irregularity of the basal layer. Discrete subepithelial edema (arrow) (G2-M3-HE×100)
Figure 5 Epithelium were similar to those of the G1(M3-M5) (G2-M4-HE×100)
Figure 6 Epithelium was similar G1 (M3-M5). Where was no subepithelial edema (G2-M5-HE×100)
At the later time, the regenerated conjunctivae epithelium was similar to the normal one, except for a smaller number of globet cells. Since the globet cells are responsible for the mucous layer of the lacrimal film, we may expect deficient mucous secretion during the conjunctivae regeneration. There are other studies about 5-FU using cell culture which demonstrated that fibroblasts treated for 5 minutes with the antimetabolites may still influence the behaviour of other cells via soluble mediators with implications in scarring after glaucoma filtration surgery [7].
Our results allowed to conclude that 5-FU at the concentration of 25g/L in the region of the corneoscleral limbus has no deleterious effects on adjacent ocular tissues. This drug may be a useful for the postoperative treatment of pterygium in order to reduce recurrence or in the neoplasia treatment.
【参考文献】
1 Mastropasqua L, Ciancaglini M, Lobefalo L, Gallenga PE. Effectiveness of intraoperative mitomycin C in the treatment of recurrent pterygium. Ophthalmologica ,1994;208:247-249
2 Cardillo JA, Alves MR, Ambrosio LE, Poterio MB. José NK. Single intraoperative application versus postoperative mitomycin C eye drops in pterygium surgery. Ophthalmology ,1995;102:1949-1952
3 Morrow GL, Stein RM, Heathcote JG, Ikeda-Douglas JV, Feldman F. Ocular toxicity of mitomycin C and 5-fluorouracil in the rabbit. Can J Ophthalmol,1994;29:268-273
4 Yeatts RP, Ford JG, Stanton CA, Reed JW. Topical 5-fluorouracil in the intraepithelial neoplasia of the conjunctiva and cornea. Ophthalmology ,1995;102:1338-1344
5 Ando H, Ido-Kawai Y, Yamamoto T, Kitazawa Y. Inhibition of corneal epithelial wound healing. A comparative study of mitomycin C and 5-fluorouracil. Ophthalmology ,1992;99:1809-1814
6 Mohay J, Mc Laughin BJ. Corneal endothelial wound repair in normal and mitotically inhibited cultures. Graefes Arch Clin Exp Ophthalmol ,1995;233:727-736
7 Daniels JT, Occleston NL, Crowston JG, Khaw PT. Effects of antimetabolite induced cellular growth arrest on fibroblasts interactions. Exp Eye Res ,1999;69:117-127
医学系护理毕业论文-长期氧疗的护理
小梁切除手术前后角膜内皮细胞观察