春季卡他性结膜炎患者泪液β2微球蛋白的测定及意义(1)

                  作者：杨国华　霍国庆　张小平　黄红 

【摘要】  　　目的：探讨春季卡他性结膜炎急性期泪液 β2微球蛋白的分泌状况。方法：选择2006-07/2006-08在解放军第546医院眼科门诊依次就诊的患者27例(50眼)，排除其他类型的结膜炎，用5mm直径滤纸采集泪液，用RIA法检测 β2 -微球蛋白的含量，并与正常对照组进行比较。结果：春卡组泪液 β2微球蛋白含量为17.71±13.50mg/L，其范围为：6.28～60.25mg/L,与同一地区正常组比较P <0.05。结论：春季卡他性结膜炎急性期 β2 -微球蛋白处于高度活化分泌状态，对探讨春季卡他性结膜炎的发病机制和指导治疗具有积极意义。

【关键词】  春季卡他性结膜炎　β2微球蛋白

　　
    The determination and significance of β2-microglobulin in vernal conjunctivitis

      Abstract AIM: To investigate secretion state of β2-microglobulin in acute stage of vernal conjunctivitis.  METHODS: This study involved Fifty eyes of 27 consecutive vernal conjunctivitis patients were involved who had been admitted by the Department of Ophthalmology, Hospital of Chinese PLA between July 2006 and August 2006. All the other categories of conjunctivitis were excluded. The patients' tears were gathered by 5-mm-diameter filter paper. The contents of β2-microglobulin were detected by RIA method and compared with that of normal control group in the same district. RESULT: The contents of β2-microglobulin of the patient were 17.71±13.50mg/L (6.28-60.25mg/L), which was significantly different from that in the controls (P <0.05). CONCLUSION: The contents of β2-microglobulin in vernal conjunctivitis patients were in activated secretor state, which has positive significance in exploring the pathogenesis and guiding the therapy of vernal conjunctivitis.

    · KEYWORDS: vernal conjunctivitis; β2-microglobulin

　　0引言

    春季卡他性结膜炎是变态反应性疾病，季节性很强，常侵犯双眼[1，2]。为了探讨春季卡他性结膜炎的发生发展规律，我们测定50眼睑结膜型春季卡他性结膜炎患者泪液中β2-微球蛋白（β2-MG），并与同一地区正常人进行比较，以探讨其与春季卡他性结膜炎关系及意义。

    1对象和方法

    1.1对象 本组27例50眼，均为急性期。男17例，女10例。年龄15～48（平均27.7±8.7）岁。双眼者23例，单眼4例（右眼2例，左眼2例）。春季卡他性结膜炎的诊断符合《眼科全书》[3]及《Wills 眼科手册第4版》[4]的标准。本组全部病例属于其中的睑结膜型。

    1.2方法 采用杭州新华滤纸厂生产的中速定量滤纸，环钻取5mm直径纸片，置玻璃皿内高压消毒后备用。用无齿结膜镊各置一备用滤纸片在双眼颞下方球结膜上，嘱受试者向鼻上象限注视，待纸片完全湿透后(一般约3～４min)，用无齿结膜镊取出，置入7cm长消毒试管内，加入0.01mol/L、pH7.4的磷酸盐缓冲液0.5mL于4℃冰箱浸泡保存24h后采用RIA法检测，其结果乘以125，即为泪液含量。 5cm直径中速定量滤纸片完全湿透饱和后，泪液含量约为4μL[3]。Fj-2008Pγ放射免疫计数器由西安262厂生产的，测定用的β2-MG试剂盒，由北京生物技术研究所提供。本地区泪液β2-MG正常值为11.35±2.98mg/L[5]。

    统计学处理：采用PEMS3.1进行统计。

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