蛋白质组学技术新进展(2)-找论文网

　　5 化学喷墨印迹

　　化学喷墨印迹是一种结合了2DE技术和蛋白质芯片技术双重优点的新型技术[22]，直接将2DE分离结果转印到膜上，形成一个固相蛋白质阵列，然后利用特殊装置对选定的蛋白质点微小部分进行原位消化，采用压电脉冲技术，无接触式微量喷进质谱完成分析。该方法省去了2DE分离后胶内酶切的多个步骤，而且经2DE分离的蛋白质可以应用多种酶消化，联合进行蛋白质序列的鉴定。化学喷墨技术是发现新的诊断标记物和药物靶点的重要工具。

　　蛋白质组学技术本身在今后数年仍将快速发展，而且会向更高的自动化方向发展。蛋白质组学将推动对疾病本质的认识，协助解决复杂疾病包括肿瘤的预防、诊断、治疗和预后判定问题。

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